Invitrogen Life Technologies Fisher Evos FL FITC Mikroskop

Invitrogen Life Technologies Fisher Evos FL FITC Mikroskop

1. What is the EVOS FL FITC microscope?

The EVOS FL is an inverted fluorescence microscope that allows researchers to observe cells and fluorescently labeled samples. The FITC channel is particularly useful for detecting green fluorescence.

• Manufacturer: Invitrogen / Life Technologies (Thermo Fisher Scientific)

• Type: Inverted fluorescence microscope

• Fluorescence channel: FITC (green fluorescence)

• Applications: Cell biology, immunofluorescence, cell viability assays, and protein localization

• Imaging: Digital imaging of fluorescent and conventional brightfield samples, depending on configuration

2. FITC fluorescence specifications

Parameter	Typical FITC values
Excitation peak	~495 nm
Emission peak	~519 nm
Excitation light	Blue
Observed fluorescence	Green
Compatible EVOS filter	GFP or FITC-specific light cube

The EVOS FL’s GFP light cube uses approximately 470 nm excitation and 510 nm emission filters. It is suitable for FITC imaging, although a dedicated FITC filter cube may provide a better spectral match.

Thermo Fisher

Fisher Scientific

3. Main features

• Inverted design: Convenient for observing living cells in culture dishes and multiwell plates.

• Fluorescence imaging: Allows detection of fluorescently labeled cells and structures.

• Brightfield and phase contrast: Enables observation of cells without fluorescent staining.

• Digital camera: Captures images for documentation and analysis.

• Integrated software: Supports image acquisition, cell counting and other imaging tasks, depending on the configuration.