BD Biosciences FACSVerse Flow Cytometer
The BD FACSVerse Flow Cytometer offers users powerful, reliable, varied cell analysis of up to 10 different parameters (8 fluorescence and 2 scatter) with robust results and signal resolution. This multicolor flow cytometer has three lasers, blue red, and violet, and 8 colors. This flow cytometer is very user-friendly, guiding users through system set up, data acquisition and analysis, straight through to shutting the system down. These start-up guides for this flow cytometer help users navigate managing configuration, qualifying performance, correction of spillover, and characterizing fluorescence. The BD FACSVerse Flow Cytometer uses streamlined software to offer predefined assays, or allow users to create their own using them as a jumping-off point. This flow cytometer system is constantly monitoring itself to make sure it’s operating at peak performance and alerting users to a potential issue before it becomes a problem.
The BD FACSVerse Flow Cytometer features a unique optical system that employs 3 free-space lasers, designed to minimize light loss and pump up a resolution in multicolor applications. This flow cytometry system also uses automated laser alignment, smart filter mirrors with built-in memory chips, and a stainless flow cell that reduces bubbles and thermal expansion. The BD FACSVerse analyzer employs a vacuum-based fluidic system that allows users total freedom in manual sample acquisition from nearly any tube configuration. This fluidics system has two modes, a standard low, medium, high sample flow rate, and a high sensitivity flow rate for dimly stained particles.
The BD FACSVerse Flow Cytometer utlizes a SIT design made up of a stainless outer probe and a high-performance polymer inner probe to draw in samples, even with smaller diameter formats. Samples for this flow cytometer can be input in a wide variety of formats, from 500ul microcentrifuge tubes to 50mL tubes all designed to fit in the same compact space. After acquisitions are complete, the sample injection tube is flushed to reduce carryover.
| Manufacturer | BD BioSciences |
| Model | FACSVerse |
| Mfg. | January 2011 |
| Catalog No. | 651155 |
| Serial No. | |
| Performance | |
| Nominal acquisition rate | 35,000 events per second |
| Carryover | Less than or equal to 0.5% |
| Sensitivity | Nominal fluorescence sensitivity in Normal mode FITC: 100 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: 25 molecules of equivalent soluble fluorochrome (MESF-PE) APC: 50 molecules of equivalent soluble fluorochrome (MESF-APC) |
| Fluorescence resolution | Coefficient of variation for PI: Area of <3%, full G0/G1 peak for propidium iodid (PI)-stained chicken erythrocyte nuclei (CEN) |
| Fluorescence linearity | Doublet/singlet ratio of 1.95-2.05 for CEN stained with PI and excited with the 488-nm (blue) laser |
| Fluidics | |
| Flow cell | Stainless stell with low coefficient of thermal expansion for predictable, stable performance |
| Cuvette internal cross-section | 430um x 180um |
| Sample flow rates | Low: 12ul/min
Medium: 60ul/min High: 120ul/min High sensitivity: 40-55ul/min |
| Fluid capacity | Standard 5-L tanks, optional 10-L tanks, 20-L sheath cubitainer adapter available |
| Sheath core stream fluid velocity | Normal: 5.4 m/s
High sensitivity: 2.7 m/s |
| Sheath fluid consumption | Normal: 13.6 mL/min
High sensitivity: 6.6 mL/min |
| Integrated cleaning cycles | Daily Clean, Monthly Clean, SIT flush |
| Other Specifications | |
| Power specifications | 100-240VAC, 50-60Hz, 2A, 150W |
| Dimensions (W x D x H) |
33 x 23 x 23.5 in. |
| Weight | 121 lb (55 kg) |
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